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biotin conjugated jacalin  (Vector Laboratories)


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    Structured Review

    Vector Laboratories biotin conjugated jacalin
    (A) Expression of CD62L and KLRG1 on primary and tertiary memory P14 CD8+ T cells 90 days post-LCMV infection and (B) binding to E- <t>and</t> <t>P-selectin.</t> (C,D) Quantification of (B). (E) Expression of CD122 and CD132. (F,G) Quantification of (E). (H) Expression of total core 1 O-glycans <t>(Jacalin)</t> and unsialylated core 1 O-glycans (PNA). (I,J) Quantification of (H). (K) Primary and tertiary memory P14 CD8+ T cells were purified and stimulated with the indicated concentration of IL-15 for 15 minutes and phosphorylation of STAT5 (Y694) was analyzed by immunoblot. (L) Primary and tertiary memory CD8+ T cells were purified and cultured with IL-15 for 3 days. Binding of MAL II, PNA and the 1B11 antibody was analyzed. (M) Same as (L) except the glycosylation of PSGL-1 was analyzed by immunoblot. (N) Same as (L) except binding to E-selectin was analyzed by flow cytometry. (O) Quantification of (N) from 4 independent experiments. (P,Q) Same as (N,O) except for P-selectin.
    Biotin Conjugated Jacalin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin conjugated jacalin/product/Vector Laboratories
    Average 93 stars, based on 49 article reviews
    biotin conjugated jacalin - by Bioz Stars, 2026-03
    93/100 stars

    Images

    1) Product Images from "Enzymatic Synthesis of Core 2 O-Glycans Governs the Tissue-Trafficking Potential of Memory CD8 + T Cells"

    Article Title: Enzymatic Synthesis of Core 2 O-Glycans Governs the Tissue-Trafficking Potential of Memory CD8 + T Cells

    Journal: Science immunology

    doi: 10.1126/sciimmunol.aan6049

    (A) Expression of CD62L and KLRG1 on primary and tertiary memory P14 CD8+ T cells 90 days post-LCMV infection and (B) binding to E- and P-selectin. (C,D) Quantification of (B). (E) Expression of CD122 and CD132. (F,G) Quantification of (E). (H) Expression of total core 1 O-glycans (Jacalin) and unsialylated core 1 O-glycans (PNA). (I,J) Quantification of (H). (K) Primary and tertiary memory P14 CD8+ T cells were purified and stimulated with the indicated concentration of IL-15 for 15 minutes and phosphorylation of STAT5 (Y694) was analyzed by immunoblot. (L) Primary and tertiary memory CD8+ T cells were purified and cultured with IL-15 for 3 days. Binding of MAL II, PNA and the 1B11 antibody was analyzed. (M) Same as (L) except the glycosylation of PSGL-1 was analyzed by immunoblot. (N) Same as (L) except binding to E-selectin was analyzed by flow cytometry. (O) Quantification of (N) from 4 independent experiments. (P,Q) Same as (N,O) except for P-selectin.
    Figure Legend Snippet: (A) Expression of CD62L and KLRG1 on primary and tertiary memory P14 CD8+ T cells 90 days post-LCMV infection and (B) binding to E- and P-selectin. (C,D) Quantification of (B). (E) Expression of CD122 and CD132. (F,G) Quantification of (E). (H) Expression of total core 1 O-glycans (Jacalin) and unsialylated core 1 O-glycans (PNA). (I,J) Quantification of (H). (K) Primary and tertiary memory P14 CD8+ T cells were purified and stimulated with the indicated concentration of IL-15 for 15 minutes and phosphorylation of STAT5 (Y694) was analyzed by immunoblot. (L) Primary and tertiary memory CD8+ T cells were purified and cultured with IL-15 for 3 days. Binding of MAL II, PNA and the 1B11 antibody was analyzed. (M) Same as (L) except the glycosylation of PSGL-1 was analyzed by immunoblot. (N) Same as (L) except binding to E-selectin was analyzed by flow cytometry. (O) Quantification of (N) from 4 independent experiments. (P,Q) Same as (N,O) except for P-selectin.

    Techniques Used: Expressing, Infection, Binding Assay, Purification, Concentration Assay, Western Blot, Cell Culture, Flow Cytometry



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    Image Search Results


    (A) Expression of CD62L and KLRG1 on primary and tertiary memory P14 CD8+ T cells 90 days post-LCMV infection and (B) binding to E- and P-selectin. (C,D) Quantification of (B). (E) Expression of CD122 and CD132. (F,G) Quantification of (E). (H) Expression of total core 1 O-glycans (Jacalin) and unsialylated core 1 O-glycans (PNA). (I,J) Quantification of (H). (K) Primary and tertiary memory P14 CD8+ T cells were purified and stimulated with the indicated concentration of IL-15 for 15 minutes and phosphorylation of STAT5 (Y694) was analyzed by immunoblot. (L) Primary and tertiary memory CD8+ T cells were purified and cultured with IL-15 for 3 days. Binding of MAL II, PNA and the 1B11 antibody was analyzed. (M) Same as (L) except the glycosylation of PSGL-1 was analyzed by immunoblot. (N) Same as (L) except binding to E-selectin was analyzed by flow cytometry. (O) Quantification of (N) from 4 independent experiments. (P,Q) Same as (N,O) except for P-selectin.

    Journal: Science immunology

    Article Title: Enzymatic Synthesis of Core 2 O-Glycans Governs the Tissue-Trafficking Potential of Memory CD8 + T Cells

    doi: 10.1126/sciimmunol.aan6049

    Figure Lengend Snippet: (A) Expression of CD62L and KLRG1 on primary and tertiary memory P14 CD8+ T cells 90 days post-LCMV infection and (B) binding to E- and P-selectin. (C,D) Quantification of (B). (E) Expression of CD122 and CD132. (F,G) Quantification of (E). (H) Expression of total core 1 O-glycans (Jacalin) and unsialylated core 1 O-glycans (PNA). (I,J) Quantification of (H). (K) Primary and tertiary memory P14 CD8+ T cells were purified and stimulated with the indicated concentration of IL-15 for 15 minutes and phosphorylation of STAT5 (Y694) was analyzed by immunoblot. (L) Primary and tertiary memory CD8+ T cells were purified and cultured with IL-15 for 3 days. Binding of MAL II, PNA and the 1B11 antibody was analyzed. (M) Same as (L) except the glycosylation of PSGL-1 was analyzed by immunoblot. (N) Same as (L) except binding to E-selectin was analyzed by flow cytometry. (O) Quantification of (N) from 4 independent experiments. (P,Q) Same as (N,O) except for P-selectin.

    Article Snippet: Lectin and Selectin Binding Fluorescein or biotin-conjugated Jacalin, Peanut Agglutinin (PNA), and MAL II (VectorLabs) were incubated with cells for 30 minutes in 1% FBS/PBS at room temperature.

    Techniques: Expressing, Infection, Binding Assay, Purification, Concentration Assay, Western Blot, Cell Culture, Flow Cytometry